Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model

Background Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions size, changes shape, and resorption of the cartilage late post-graft period. study aimed to investigate effects adipose-derived mesenchymal cells conditioned medium (CM) on viability.Methods Dissections were performed 4 months after injection 0.5 mL CM or 2×10<sup>6</sup> stem (MSCs) grafting into a control group two experimental groups (n=21 rabbits total). Chondrocyte viability type II collagen expression grafted areas analyzed hematoxylin-eosin staining immunohistochemical methods, respectively.Results In MSC groups, chondrocyte proliferation at tissue incision margin (MSC: P<0.01, CM: P<0.0001), auricular P<0.05, integration with surrounding P<0.001, P<0.0001) levels P=0.001, P=0.0002) significantly increased.Conclusions Xenogenic MSCs contributed new production without any tumoral immune reactions. particular, cell-free nature strengthened its potential for safe use. injections can preserve interest this technique is expected increase long-term results from clinical studies subject become available.